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Mitochondrial aldehyde dehydrogenase-2 coordinates the hydrogen sulfide - AMPK axis to attenuate high glucose-induced pancreatic β-cell dysfunction by glutathione antioxidant system - ScienceDirect

371,372 Oxidative stress and inflammation induced by elevated ROS are involved in many diabetic complications

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To compare nucleoside levels of adenosine, AMP, ADP, and ATP, 25 µL of 55% chloroacetaldehyde (Sigma-Aldrich) combined with 500 µL lysate and the solution was incubated at 72 °C for 0.5 h on a heat block with shaking (500 rpm) 73