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To test whether PIN1 can catalyze the isomerization of the region around T208-P209, we conducted a 1 H- 1 H EXchange SpectroscopY (EXSY) using a short phosphorylated RNF168 peptide, SDPV(pT)PK in the presence and absence of PIN1
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Pack Size:60 Softgels
The cleared tissue lysates were subjected to Western blot analysis for glutathionylated protein with anti-GSH antibody (Virogen, #101-A-250, 1:500) under non-reducing condition, and other antibodies under reducing conditions: Grx1 (Abcam, ab45953, 1:1,000), GR (abclone, A4566, 1:1,000), GST-π (MLB, 312, 1:1,000) and eNOS (Cell Signaling, 1:1,000)
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