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Incubation of the crude grapefruit PMP fraction with a final concentration of 50mM EDTA (pH 7.2- 8), followed by overnight dialysis using a 300kDa membrane, successfully removed contaminants present in the late elution fractions after SEC, as shown by absorbance at 280 nm (Fig
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This enzyme “wastes” a helpful molecule called nicotinamide (a form of vitamin B3) by turning it into something useless instead of recycling it into NAD+, think of NAD+ as the main “battery fuel” that powers your cells’ energy factories (mitochondria)
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