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In this perspective, it should be noted that some studies show the occurrence of neurotoxic effects at very high doses, which are correlated with a considerable reduction in dopaminergic neuronal density in the substantia nigra [122], data that are confirmed by other studies with a decrease in the density of serotonergic and cholinergic neurons [123]

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Unlike many other vitamins, B12 is stored in the liver in substantial quantities, meaning deficiency typically develops gradually over months to years as these reserves become depleted