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Biphasic concentration–response curves were fitted with a combination of two sigmoid saturation curves of the form where X is the logarithm of the free Zn 2+ concentration that produces the effect Y, A 1 and A 2 are the bottom and top asymptotes, K Zn1 and K Zn2 the free Zn 2+ concentrations producing 50% of the E max is mediated by the two components, h 1 and h 2 the corresponding Hill slopes, and p the proportion of maximum response due to the more potent component

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