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Methods Cell lines and reagents HEK293T and HeLa cells were maintained in high-glucose DMEM (Sigma-Aldrich, D6429) containing 10% fetal calf serum (Sigma-Aldrich, P4333), 100 U ml −1 penicillin and 100 µg ml −1 streptomycin at 37 °C and 5% CO 2
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Decreasing levels of estrogen may cause the hypothalamus (the part of the brain that acts as the body’s thermostat) to become more sensitive to changes in body temperature
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