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A quick checklist to keep next to the bench [ ] Vial at room temperature before opening [ ] Both septa sanitised with 70% IPA and dried [ ] Correct solvent selected (BAC water for multi-use, SWFI for single-use) [ ] Reconstitution volume calculated to hit a working concentration you can pipette accurately [ ] Solvent added down the glass wall, not directly onto the cake [ ] Swirled, never shaken [ ] Solution inspected for clarity — no haze, no particulates [ ] Vial labelled with peptide, concentration, solvent, date, initials [ ] Stored at 2–8°C immediately [ ] Shelf-life window logged in the lab notebook Final note on quality All the reconstitution technique in the world can’t fix a poor-quality peptide
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