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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 µl ice-cold PBS and fixed by adding 700 µl ice-cold absolute ethanol while gently vortexing

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[2] In principle, combining a senolytic approach through The Rapamycin Protocol with the anti-inflammatory and regenerative activity of GHK-Cu could address the aging tissue problem from two complementary angles: reducing the burden of senescent cells while simultaneously promoting the repair and regenerative activity of the remaining healthy cells