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inactive mutant forms of CDO (e.g., His86Ala, leading to loss of the His that serves as a metal ligand in the active site) did not form any cross-link, and mutant forms of CDO with low activity (e.g., Arg60Ala, which has a markedly decreased affinity for Cys, probably due to the loss of the hydrogen bonding partner for the carboxylate of the substrate) formed the cross-link more slowly
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The Metabolic Basis of Inherited Disease, 6th Edition
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