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Quantitative PCR was performed using SYBR Green PCR Master Mix (Thermo Fisher Scientific, Inc.) and the primers were as follows: COXI: Forward, 5′-ATGCGGCCATAGGTTCTGC-3′ and reverse, 5′-TCCTCAAGATGTCTCAGTTCCAT-3′
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Structural insight into the molecular mechanism of allosteric activation of human cystathionine β-synthase by S-adenosylmethionine
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