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Statistical analyses were performed with the GraphPad 5.0 software (La Jolla, CA, USA) with ANOVA non-parametrical test and then unpaired Student’s t -test

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The labeled cDNAs were used to detect marker genes of signal transduction pathways on microarray membranes using Human Signal Transduction PathwayFinder™ RT 2 Profiler™ PCR Array (SuperArray System, Bioscience, Frederick, MD, USA)