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ELISA The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein (MCP)-1 in serum and liver tissue samples were determined by ELISA kits (R&D systems) [51], The levels of TGF-β and IL10 in serum were determined by ELISA kits (R&D systems) [52]
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