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Cultures were then harvested by centrifugation at 5000 g for 10 min before removing supernatant and resuspending the resulting cell pellet in 1 mL of lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, mini cOmplete protease inhibitor (Roche, Basel, Switzerland), 20 μg/mL DNase I, and 20 μg/mL RNase A)

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Size:30-Count Bottle

[Google Scholar] 144.Zhou Y., Liu W.Y., Cao W.D., Cheng Y., Liu Z., Chen X.Q