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Analysis of the interaction of antimalarial agents with Plasmodium falciparum glutathione reductase through molecular mechanical calculations | Journal of Molecular Modeling | Springer Nature Link

When to Discard Even If It Looks Fine Discard the vial under any of these conditions, regardless of appearance: More than 28 days since first puncture More than 15 punctures through the stopper Vial was left at room temperature for more than 48 hours after opening Vial was exposed to temperatures above 40°C after opening Vial was frozen and thawed You are unsure when it was first opened Bacteriostatic water is inexpensive

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A measure of 50 µL of stop solution was added to each well, and after 10 to 20 min, the plate was quickly inspected at 450 nm using a plate reader (Infinite 200 Pro MPlex, Tecan, Mannedorf, Switzerland)

with each pad absorbing about 2.5 ml of solution