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Analytical ultracentrifugation Hexanucleosomes bound to linker histones H1.4 (WT, RS and RSΔC) were prepared as described above and dialyzed overnight against a buffer containing (i) 5 mM Tris-HCl pH 7.4, 0.25 mM EDTA and either 50 or 90 mM NaCl or (ii) 5 mM Tris-HCl pH 7.4 and either 0.35, 0.5 or 0.6 mM MgCl 2

Glycin

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In reaction mixture without enzyme solution, varying amounts of glutathione (0-300 μl of stock solution giving 0-260 μM final glutathione concentrations) were reacted with 150 μl of monobromobimane solution (final concentration 50 μM) in presence of phosphate buffer (3.0 ml) in cuvettes